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4 records – page 1 of 1.

The characterization of ADAMTS-12 in the regulation of human trophoblast invasion in vitro

https://cwslc.andornot.com/en/permalink/catalog113949
Zou, Junxuan. [Vancouver, BC?]: University of British Columbia , 2010.
Website
http://hdl.handle.net/2429/29783
Material Type
Thesis
Call Number
Thesis Shelf
The distintegrin-like and metalloproteinase with thrombospondin repeats (ADAMTS) are members of a gene family of secreted, multidomain and multifunctional proteinases that are able to proteolytically degrade a diverse array of cellular, extracellular and extracellular matrix (ECM) substrates. We ex…
Website
http://hdl.handle.net/2429/29783
Author
Zou, Junxuan
Place of Publication
[Vancouver, BC?]
Publisher
University of British Columbia
Publication Date
2010
Subject
Faculty of Medicine
Theses
Abstract
The distintegrin-like and metalloproteinase with thrombospondin repeats (ADAMTS) are members of a gene family of secreted, multidomain and multifunctional proteinases that are able to proteolytically degrade a diverse array of cellular, extracellular and extracellular matrix (ECM) substrates. We examined the presence of ADAMTS in first trimester human placenta and only ADAMTS-12 was present in cultures of invasive extravillous cytotrophoblast (EVT) at significantly higher levels than in poorly invasive JEG-3 chorocarcinoma cells. Immunohistochemistry staining of chorionic villi derived from first trimester human placenta demonstrated that ADAMTS-12 was intensively immunolocalized in the cytotrophoblast layer but weakly immunolocalized in the poorly-invasive syncytial trophoblast layer.
Language
English
Material Type
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Human gonadotropin-releasing hormone-II regulation in ovarian cancer : mechanisms and potential functional role

https://cwslc.andornot.com/en/permalink/catalog113953
Poon, Song Ling. [Vancouver, BC?]: University of British Columbia , 2010.
Website
http://hdl.handle.net/2429/30021
Material Type
Thesis
Call Number
Thesis Shelf
It is increasingly apparent that GnRH-II acts as an autocrine/paracrine regulator in non-pituitary tissues, in addition to its role in the regulation of gonadotropin synthesis, and is an important player in cancer cell biology. High levels of GnRH-II and GnRHR in malignant ovarian tumors as compare…
Website
http://hdl.handle.net/2429/30021
Author
Poon, Song Ling
Place of Publication
[Vancouver, BC?]
Publisher
University of British Columbia
Publication Date
2010
Subject
Faculty of Medicine
Theses
Abstract
It is increasingly apparent that GnRH-II acts as an autocrine/paracrine regulator in non-pituitary tissues, in addition to its role in the regulation of gonadotropin synthesis, and is an important player in cancer cell biology. High levels of GnRH-II and GnRHR in malignant ovarian tumors as compared with benign ovarian tissues underlies the importance of understanding GnRH-II function in ovarian cancer. In an attempt to define the regulation of GnRH-II in these tissues, we found that a cyclic-AMP responsive element (CRE) is critical for GnRH-II promoter transcription.
Language
English
Material Type
Thesis
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Noninvasive assessment of embryo quality in human in vitro fertilization : metabolomic profiling of embryo culture media with Raman spectroscopy

https://cwslc.andornot.com/en/permalink/catalog113946
Asghari Roodsari, Alaleh. [Vancouver, BC?]: University of British Columbia , 2012.
Website
http://hdl.handle.net/2429/43273
Material Type
Thesis
Call Number
Thesis Shelf
Light microscopy has remained the primary tool for the assessment of embryo quality and the selection of embryos for transfer in clinical IVF practice. Recent studies have suggested that metabolomic profiling of embryo culture media can distinguish human embryos with better implantation potential. …
Website
http://hdl.handle.net/2429/43273
Author
Asghari Roodsari, Alaleh
Place of Publication
[Vancouver, BC?]
Publisher
University of British Columbia
Publication Date
2012
Subject
Faculty of Medicine
Theses
Abstract
Light microscopy has remained the primary tool for the assessment of embryo quality and the selection of embryos for transfer in clinical IVF practice. Recent studies have suggested that metabolomic profiling of embryo culture media can distinguish human embryos with better implantation potential. We therefore undertook the following study to further assess the usefulness of metabolomic profiling ?spent? embryo culture medium using Raman spectroscopy. Methods: Patients undergoing IVF+/-ICSI treatment from the UBC Centre for Reproductive Health were recruited for study.
Language
English
Material Type
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Effects and mechanisms of growth differentiation factor 9 on activin A-regulated inhibin B and progesterone production in human granulosa cells

https://cwslc.andornot.com/en/permalink/catalog113951
Shi, Fengtao. [Vancouver, BC?]: University of British Columbia , 2010.
Website
http://hdl.handle.net/2429/28961
Material Type
Thesis
Call Number
Thesis Shelf
Activin A (homodimer of inhibin βA-subunit) is known to increase inhibin βB-subunit and inhibin B (heterodimer of inhibin α- and βB-subunit) levels and decrease progesterone accumulation in human granulosa cells. Growth differentiation factor 9 (GDF9) is a potent paracrine regul…
Website
http://hdl.handle.net/2429/28961
Author
Shi, Fengtao
Place of Publication
[Vancouver, BC?]
Publisher
University of British Columbia
Publication Date
2010
Subject
Faculty of Medicine
Theses
Abstract
Activin A (homodimer of inhibin βA-subunit) is known to increase inhibin βB-subunit and inhibin B (heterodimer of inhibin α- and βB-subunit) levels and decrease progesterone accumulation in human granulosa cells. Growth differentiation factor 9 (GDF9) is a potent paracrine regulator of ovarian function, but its overall effects, particularly relating to activin A actions, are unknown. We examined the potential crosstalk between activin A and GDF9 in primary cultures of human granulosa-lutein (hGL) cells. Pretreatment of hGL cells with GDF9 for 24 h resulted in an increased expression of activin receptors and Smad2/3, and decreased inhibitory Smad7 activity. These effects were attenuated by BMP type II receptor ectodomain (BMPR2 ECD), a GDF9 antagonist.
Language
English
Material Type
Thesis
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