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Single cell studies of calcium as second messenger in human granulosa-lutein and embryonic kidney 293 cells

https://cwslc.andornot.com/en/permalink/catalog113979
Lee, Pearly S.N. [Vancouver, BC?]: University of British Columbia , 1999.
Website
http://hdl.handle.net/2429/10789
Material Type
Thesis
Call Number
Thesis Shelf
It is well established that LH action is mediated primarily by adenylate cyclase/cAMP. Conversely, the role of inositol phosphate/calcium in LH signalling has only recently been investigated. We examined the effects of gonadotrophins on intracellular calcium mobilisation in HEK293 cells transiently…
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Website
http://hdl.handle.net/2429/10789
Author
Lee, Pearly S.N.
Place of Publication
[Vancouver, BC?]
Publisher
University of British Columbia
Publication Date
1999
Subject
Faculty of Medicine
Theses
Abstract
It is well established that LH action is mediated primarily by adenylate cyclase/cAMP. Conversely, the role of inositol phosphate/calcium in LH signalling has only recently been investigated. We examined the effects of gonadotrophins on intracellular calcium mobilisation in HEK293 cells transiently transfected with human wild type or chimeric gonadotrophs receptors (n=3400). Intracellular free calcium concentration was measured using fura-2 microspectrofluorimetric techniques. Human LH (2-4 μg/ml) arid CG (10 IU/ml) consistently evoked oscillatory calcium signals in HEK293 cells transfected with hLHr, whereas hFSH (2-4 μg/ml) failed to elicit any calcium responses.
Language
English
Material Type
Thesis
Call Number
Thesis Shelf
Websites
http://hdl.handle.net/2429/10789
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