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Single cell studies of calcium as second messenger in human granulosa-lutein and embryonic kidney 293 cells

https://cwslc.andornot.com/en/permalink/catalog113979

Lee, Pearly S.N. [Vancouver, BC?]: University of British Columbia , 1999.

Website: http://hdl.handle.net/2429/10789

Material Type: Thesis

Call Number: Thesis Shelf

It is well established that LH action is mediated primarily by adenylate cyclase/cAMP. Conversely, the role of inositol phosphate/calcium in LH signalling has only recently been investigated. We examined the effects of gonadotrophins on intracellular calcium mobilisation in HEK293 cells transiently…

1 website

Website: http://hdl.handle.net/2429/10789

Author: Lee, Pearly S.N.

Place of Publication: [Vancouver, BC?]

Publisher: University of British Columbia

Publication Date: 1999

Subject: Faculty of Medicine; Theses

Abstract: It is well established that LH action is mediated primarily by adenylate cyclase/cAMP. Conversely, the role of inositol phosphate/calcium in LH signalling has only recently been investigated. We examined the effects of gonadotrophins on intracellular calcium mobilisation in HEK293 cells transiently transfected with human wild type or chimeric gonadotrophs receptors (n=3400). Intracellular free calcium concentration was measured using fura-2 microspectrofluorimetric techniques. Human LH (2-4 μg/ml) arid CG (10 IU/ml) consistently evoked oscillatory calcium signals in HEK293 cells transfected with hLHr, whereas hFSH (2-4 μg/ml) failed to elicit any calcium responses.

Language: English

Material Type: Thesis

Call Number: Thesis Shelf

Websites

http://hdl.handle.net/2429/10789

Studies of a sperm acrosomal antigen recognized by HS-63 monoclonal antibody

https://cwslc.andornot.com/en/permalink/catalog113989

Liu, Ming-Sun. [Vancouver, BC?]: University of British Columbia , 1991.

Website: http://hdl.handle.net/2429/30996

Material Type: Thesis

Call Number: Thesis Shelf

A sperm specific and species conserved monoclonal antibody (HS-63) was shown to inhibit in vitro fertilization of mouse oocytes and human sperm penetration to zona-free hamster ova. The sperm antigen (SA-63) which reacts with HS-63 was found to be localized on the sperm acrosome. Following sperm ca…

1 website

Website: http://hdl.handle.net/2429/30996

Author: Liu, Ming-Sun

Place of Publication: [Vancouver, BC?]

Publisher: University of British Columbia

Publication Date: 1991

Subject: Faculty of Medicine; Theses

Abstract: A sperm specific and species conserved monoclonal antibody (HS-63) was shown to inhibit in vitro fertilization of mouse oocytes and human sperm penetration to zona-free hamster ova. The sperm antigen (SA-63) which reacts with HS-63 was found to be localized on the sperm acrosome. Following sperm capacitation, this antigen becomes exposed and is shed after the acrosome reaction. SA-63 may be involved in the sperm acrosome reaction during the initial fertilization process.

Language: English

Material Type: Thesis

Call Number: Thesis Shelf

Websites

http://hdl.handle.net/2429/30996