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In contrast to the success of ICSI in treating male infertility, concerns have been raised about the health outcomes of the children conceived through this procedure. Cohort studies have shown that the ICSI population has an increase in low birth weight (LBW), birth defects, chromosomal abnormaliti…
The association between subsets of male infertility, oligoasthenoteratozoospermia (OAT), obstructive azoospermic (OA), non-obstructive azoospermia (NOA), and increased risk of having chromosomal abnormalities in the sperm has not been investigated. Chromosomal aneuploidy in sperm will be systematic…
With the increasing use of assisted reproductive techniques (ART) every year, concerns have been raised regarding the possible effect these procedures have on the health of the children procured by them. Although patients born via ART are usually healthy, studies have associated these procedures wi…
In order to characterize and compare the pattern of inhibin production in the follicular phase between patients of the clomiphene citrate/human menopausal gonadotropin (hMG) and Buserelin/hMG protocols in in-vitro fertilization, serum inhibin levels from 20 patients in each protocol were measured. …
Light microscopy has remained the primary tool for the assessment of embryo quality and the selection of embryos for transfer in clinical IVF practice. Recent studies have suggested that metabolomic profiling of embryo culture media can distinguish human embryos with better implantation potential. …
A delicate balance of steroid and gonadotrophic hormones is essential for intrafollicular oocyte maturation and successful fertilization and embryonic development. Previous studies have demonstrated that a superovulatory dose of pregnant mare serum gonadotrophin (PMSG) has excessive gonadotrophic a…
Abnormal DNA methylation at imprinted and non-imprinted genes has been associated with spermatogenesis failure. However, little information is available regarding DNA methylation at those genes in men affected by severe male factor infertility. We hypothesized a higher incidence of aberrant DNA met…
There is increasing evidence to suggest that activin can function as an autocrine/paracrine regulator in various tissues, including the reproductive system. At the cellular level, activin acts via a family of activin receptor (ActR) subtypes which includes two type I (ActRI and ActRLB) and two type…
Pregnancies derived from in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) are associated with increased rates of chromosome abnormalities, congenital malformations, low birth weight, pre-term births, multiple gestations, and imprinting disorders. Assisted reproductive techno…
In vitro fertilization (IVF) has been achieved in several mammalian species, including humans. Using the technique of IVF and embryo transfer, pregnancies can now be established in infertile women and thousands of live births have occurred. However, many of the embryos transferred to the uterus fai…
The distintegrin-like and metalloproteinase with thrombospondin repeats (ADAMTS) are members of a gene family of secreted, multidomain and multifunctional proteinases that are able to proteolytically degrade a diverse array of cellular, extracellular and extracellular matrix (ECM) substrates. We ex…
Intracytoplasmic sperm injection (ICSI) has been a successful assisted reproductive technique for men with severe male-factor infertility. However, ICSI requires the subjective selection of normal looking sperm, which does not preclude the transmission of genetically abnormal sperm. Correlation bet…
In order to characterize and compare the pattern of inhibin production in the follicular phase between patients of the clomiphene citrate/human menopausal gonadotropin (hMG) and Buserelin/hMG protocols in in-vitro fertilization, serum inhibin levels from 20 patients in each protocol were measured. …
This thesis work is focused on studies of sperm antigen: its purification and antifertility effect, its "internal image" by use of anti-idiotypic antibodies, and its capacity to induce antibodies of IgA class. Mouse sperm acrosomal antigens that react with a monoclonal antibody generated against mo…
A sperm specific and species conserved monoclonal antibody (HS-63) was shown to inhibit in vitro fertilization of mouse oocytes and human sperm penetration to zona-free hamster ova. The sperm antigen (SA-63) which reacts with HS-63 was found to be localized on the sperm acrosome. Following sperm ca…
The only book of its kind,Manual of High Risk Pregnancy & Delivery provides a complete resource for care of this special patient and her complex needs. It helps you provide positive outcomes with coverage of today's newest technology, physiologic considerations, psychologic implications, health dis…
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Written by a Prenatal Clinical Ecucator, this module assists registered nurses working in the Diabetes in Pregnancy Clinic to acquire the necessary knowledge in order to perform insulin adjustment in pregnancy. This is intended as an introduction, and must be complemented by supervised clinical exp…
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